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pbluescript ii ks(-) phagemid  (Agilent technologies)


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    Structured Review

    Agilent technologies pbluescript ii ks(-) phagemid

    Pbluescript Ii Ks( ) Phagemid, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pbluescript+ii+ks(-)+phagemid/pmc06344338-89-0-5
    Average 90 stars, based on 1 article reviews
    pbluescript ii ks(-) phagemid - by Bioz Stars, 2026-10
    90/100 stars

    Images

    1) Product Images from "Mechanism of Bidirectional Leading-Strand Synthesis Establishment at Eukaryotic DNA Replication Origins"

    Article Title: Mechanism of Bidirectional Leading-Strand Synthesis Establishment at Eukaryotic DNA Replication Origins

    Journal: Molecular Cell

    doi: 10.1016/j.molcel.2018.10.019


    Figure Legend Snippet:

    Techniques Used: Recombinant, Purification, Mutagenesis, Software

    Related Articles

    Clone Assay:

    Article Title: Mechanism of Bidirectional Leading-Strand Synthesis Establishment at Eukaryotic DNA Replication Origins
    Article Snippet: pBluescript II KS(-) Phagemid , Agilent Technologies , Cat# 212208-51.

    Article Title: Unconventional gene arrangement and content revealed by full genome analysis of the white sturgeon adenovirus, the single member of the genus Ichtadenovirus.
    Article Snippet: Adenoviruses are commonly found in members of almost every vertebrate lineage except fish and amphibians, from each of which only a single isolate is available as yet.. In this work, the complete genomic sequence of a fish adenovirus, originating from the white sturgeon (Acipenser transmontanus), was determined and analyzed.. Several exceptional features were observed including the longest hitherto known genome size (of 48,395 bp) and a strange location of the putative fiber genes resulting in an unconventional organization pattern.

    Article Title: Discovery of stromal regulatory networks that suppress Ras-sensitized epithelial cell proliferation
    Article Snippet: The PCR products were digested with restriction enzymes and sequentially inserted into the pBluescript II KS(+) phagemid vector (Agilent Technologies; 212207).

    Article Title: L1 retrotransposon-mediated stable gene silencing
    Article Snippet: The neo -cassette tagged L1 RP was inserted into pBluescript II KS(−) phagemid vector (Stratagene, La Jolla, CA) to create pBSKS-L1 RP - neo .

    Article Title: Purification and Partial Characterization of an Entomopoxvirus (DlEPV) from a Parasitic Wasp of Tephritid Fruit Flies.
    Article Snippet: About 10 μg of DlEPV DNA were digested with the EcoRI (RI) restriction enzyme (Roche Molecular Biochemicals) and cloned into the EcoRI site of the pBluescript II KS (+/−) phagemid cloning vector (pBS; Stratagene, www.stratagene.com/ ) using standard protocols ( ).

    Article Title: A Novel Role of RASSF9 in Maintaining Epidermal Homeostasis
    Article Snippet: Fragments corresponding to nucleotides (nt) 285–817 and 1126–1625 of the mouse RASSF9 cDNA (NCBI RefSeq NM_146240) were separately cloned into the Eco RI- Xho I sites of the pBluescript II KS (+) phagemid vector (Stratagene, La Jolla, CA) for riboprobe synthesis.

    Article Title: Antibodies Directed toward Neuraminidase N1 Control Disease in a Mouse Model of Influenza
    Article Snippet: PCR products were purified using the Isolate II PCR and gel kit (Bioline) and cloned in the pBluescript II Phagemid KS+ vector (Agilent Technologies) by blunt-end ligation using T4 ligase (Fermentas).

    Plasmid Preparation:

    Article Title: Mechanism of Bidirectional Leading-Strand Synthesis Establishment at Eukaryotic DNA Replication Origins
    Article Snippet: pBluescript II KS(-) Phagemid , Agilent Technologies , Cat# 212208-51.

    Article Title: Unconventional gene arrangement and content revealed by full genome analysis of the white sturgeon adenovirus, the single member of the genus Ichtadenovirus.
    Article Snippet: Adenoviruses are commonly found in members of almost every vertebrate lineage except fish and amphibians, from each of which only a single isolate is available as yet.. In this work, the complete genomic sequence of a fish adenovirus, originating from the white sturgeon (Acipenser transmontanus), was determined and analyzed.. Several exceptional features were observed including the longest hitherto known genome size (of 48,395 bp) and a strange location of the putative fiber genes resulting in an unconventional organization pattern.

    Article Title: Discovery of stromal regulatory networks that suppress Ras-sensitized epithelial cell proliferation
    Article Snippet: The PCR products were digested with restriction enzymes and sequentially inserted into the pBluescript II KS(+) phagemid vector (Agilent Technologies; 212207).

    Article Title: L1 retrotransposon-mediated stable gene silencing
    Article Snippet: The neo -cassette tagged L1 RP was inserted into pBluescript II KS(−) phagemid vector (Stratagene, La Jolla, CA) to create pBSKS-L1 RP - neo .

    Article Title: Purification and Partial Characterization of an Entomopoxvirus (DlEPV) from a Parasitic Wasp of Tephritid Fruit Flies.
    Article Snippet: About 10 μg of DlEPV DNA were digested with the EcoRI (RI) restriction enzyme (Roche Molecular Biochemicals) and cloned into the EcoRI site of the pBluescript II KS (+/−) phagemid cloning vector (pBS; Stratagene, www.stratagene.com/ ) using standard protocols ( ).

    Article Title: A Novel Role of RASSF9 in Maintaining Epidermal Homeostasis
    Article Snippet: Fragments corresponding to nucleotides (nt) 285–817 and 1126–1625 of the mouse RASSF9 cDNA (NCBI RefSeq NM_146240) were separately cloned into the Eco RI- Xho I sites of the pBluescript II KS (+) phagemid vector (Stratagene, La Jolla, CA) for riboprobe synthesis.

    Article Title: Antibodies Directed toward Neuraminidase N1 Control Disease in a Mouse Model of Influenza
    Article Snippet: PCR products were purified using the Isolate II PCR and gel kit (Bioline) and cloned in the pBluescript II Phagemid KS+ vector (Agilent Technologies) by blunt-end ligation using T4 ligase (Fermentas).

    Sequencing:

    Article Title: Mechanism of Bidirectional Leading-Strand Synthesis Establishment at Eukaryotic DNA Replication Origins
    Article Snippet: pBluescript II KS(-) Phagemid , Agilent Technologies , Cat# 212208-51.

    Article Title: Unconventional gene arrangement and content revealed by full genome analysis of the white sturgeon adenovirus, the single member of the genus Ichtadenovirus.
    Article Snippet: Adenoviruses are commonly found in members of almost every vertebrate lineage except fish and amphibians, from each of which only a single isolate is available as yet.. In this work, the complete genomic sequence of a fish adenovirus, originating from the white sturgeon (Acipenser transmontanus), was determined and analyzed.. Several exceptional features were observed including the longest hitherto known genome size (of 48,395 bp) and a strange location of the putative fiber genes resulting in an unconventional organization pattern.

    Article Title: Discovery of stromal regulatory networks that suppress Ras-sensitized epithelial cell proliferation
    Article Snippet: The PCR products were digested with restriction enzymes and sequentially inserted into the pBluescript II KS(+) phagemid vector (Agilent Technologies; 212207).

    Article Title: L1 retrotransposon-mediated stable gene silencing
    Article Snippet: The neo -cassette tagged L1 RP was inserted into pBluescript II KS(−) phagemid vector (Stratagene, La Jolla, CA) to create pBSKS-L1 RP - neo .

    Article Title: Purification and Partial Characterization of an Entomopoxvirus (DlEPV) from a Parasitic Wasp of Tephritid Fruit Flies.
    Article Snippet: About 10 μg of DlEPV DNA were digested with the EcoRI (RI) restriction enzyme (Roche Molecular Biochemicals) and cloned into the EcoRI site of the pBluescript II KS (+/−) phagemid cloning vector (pBS; Stratagene, www.stratagene.com/ ) using standard protocols ( ).

    Article Title: A Novel Role of RASSF9 in Maintaining Epidermal Homeostasis
    Article Snippet: Fragments corresponding to nucleotides (nt) 285–817 and 1126–1625 of the mouse RASSF9 cDNA (NCBI RefSeq NM_146240) were separately cloned into the Eco RI- Xho I sites of the pBluescript II KS (+) phagemid vector (Stratagene, La Jolla, CA) for riboprobe synthesis.

    Article Title: Antibodies Directed toward Neuraminidase N1 Control Disease in a Mouse Model of Influenza
    Article Snippet: PCR products were purified using the Isolate II PCR and gel kit (Bioline) and cloned in the pBluescript II Phagemid KS+ vector (Agilent Technologies) by blunt-end ligation using T4 ligase (Fermentas).

    Molecular Cloning:

    Article Title: Mechanism of Bidirectional Leading-Strand Synthesis Establishment at Eukaryotic DNA Replication Origins
    Article Snippet: pBluescript II KS(-) Phagemid , Agilent Technologies , Cat# 212208-51.

    Article Title: Unconventional gene arrangement and content revealed by full genome analysis of the white sturgeon adenovirus, the single member of the genus Ichtadenovirus.
    Article Snippet: Adenoviruses are commonly found in members of almost every vertebrate lineage except fish and amphibians, from each of which only a single isolate is available as yet.. In this work, the complete genomic sequence of a fish adenovirus, originating from the white sturgeon (Acipenser transmontanus), was determined and analyzed.. Several exceptional features were observed including the longest hitherto known genome size (of 48,395 bp) and a strange location of the putative fiber genes resulting in an unconventional organization pattern.

    Article Title: Discovery of stromal regulatory networks that suppress Ras-sensitized epithelial cell proliferation
    Article Snippet: The PCR products were digested with restriction enzymes and sequentially inserted into the pBluescript II KS(+) phagemid vector (Agilent Technologies; 212207).

    Article Title: L1 retrotransposon-mediated stable gene silencing
    Article Snippet: The neo -cassette tagged L1 RP was inserted into pBluescript II KS(−) phagemid vector (Stratagene, La Jolla, CA) to create pBSKS-L1 RP - neo .

    Article Title: Purification and Partial Characterization of an Entomopoxvirus (DlEPV) from a Parasitic Wasp of Tephritid Fruit Flies.
    Article Snippet: About 10 μg of DlEPV DNA were digested with the EcoRI (RI) restriction enzyme (Roche Molecular Biochemicals) and cloned into the EcoRI site of the pBluescript II KS (+/−) phagemid cloning vector (pBS; Stratagene, www.stratagene.com/ ) using standard protocols ( ).

    Article Title: A Novel Role of RASSF9 in Maintaining Epidermal Homeostasis
    Article Snippet: Fragments corresponding to nucleotides (nt) 285–817 and 1126–1625 of the mouse RASSF9 cDNA (NCBI RefSeq NM_146240) were separately cloned into the Eco RI- Xho I sites of the pBluescript II KS (+) phagemid vector (Stratagene, La Jolla, CA) for riboprobe synthesis.

    Article Title: Antibodies Directed toward Neuraminidase N1 Control Disease in a Mouse Model of Influenza
    Article Snippet: PCR products were purified using the Isolate II PCR and gel kit (Bioline) and cloned in the pBluescript II Phagemid KS+ vector (Agilent Technologies) by blunt-end ligation using T4 ligase (Fermentas).

    Recombinant:

    Article Title: Mechanism of Bidirectional Leading-Strand Synthesis Establishment at Eukaryotic DNA Replication Origins
    Article Snippet: pBluescript II KS(-) Phagemid , Agilent Technologies , Cat# 212208-51.

    Article Title: Unconventional gene arrangement and content revealed by full genome analysis of the white sturgeon adenovirus, the single member of the genus Ichtadenovirus.
    Article Snippet: Adenoviruses are commonly found in members of almost every vertebrate lineage except fish and amphibians, from each of which only a single isolate is available as yet.. In this work, the complete genomic sequence of a fish adenovirus, originating from the white sturgeon (Acipenser transmontanus), was determined and analyzed.. Several exceptional features were observed including the longest hitherto known genome size (of 48,395 bp) and a strange location of the putative fiber genes resulting in an unconventional organization pattern.

    Article Title: Discovery of stromal regulatory networks that suppress Ras-sensitized epithelial cell proliferation
    Article Snippet: The PCR products were digested with restriction enzymes and sequentially inserted into the pBluescript II KS(+) phagemid vector (Agilent Technologies; 212207).

    Article Title: L1 retrotransposon-mediated stable gene silencing
    Article Snippet: The neo -cassette tagged L1 RP was inserted into pBluescript II KS(−) phagemid vector (Stratagene, La Jolla, CA) to create pBSKS-L1 RP - neo .

    Article Title: Purification and Partial Characterization of an Entomopoxvirus (DlEPV) from a Parasitic Wasp of Tephritid Fruit Flies.
    Article Snippet: About 10 μg of DlEPV DNA were digested with the EcoRI (RI) restriction enzyme (Roche Molecular Biochemicals) and cloned into the EcoRI site of the pBluescript II KS (+/−) phagemid cloning vector (pBS; Stratagene, www.stratagene.com/ ) using standard protocols ( ).

    Article Title: A Novel Role of RASSF9 in Maintaining Epidermal Homeostasis
    Article Snippet: Fragments corresponding to nucleotides (nt) 285–817 and 1126–1625 of the mouse RASSF9 cDNA (NCBI RefSeq NM_146240) were separately cloned into the Eco RI- Xho I sites of the pBluescript II KS (+) phagemid vector (Stratagene, La Jolla, CA) for riboprobe synthesis.

    Article Title: Antibodies Directed toward Neuraminidase N1 Control Disease in a Mouse Model of Influenza
    Article Snippet: PCR products were purified using the Isolate II PCR and gel kit (Bioline) and cloned in the pBluescript II Phagemid KS+ vector (Agilent Technologies) by blunt-end ligation using T4 ligase (Fermentas).

    Purification:

    Article Title: Mechanism of Bidirectional Leading-Strand Synthesis Establishment at Eukaryotic DNA Replication Origins
    Article Snippet: pBluescript II KS(-) Phagemid , Agilent Technologies , Cat# 212208-51.

    Article Title: Unconventional gene arrangement and content revealed by full genome analysis of the white sturgeon adenovirus, the single member of the genus Ichtadenovirus.
    Article Snippet: Adenoviruses are commonly found in members of almost every vertebrate lineage except fish and amphibians, from each of which only a single isolate is available as yet.. In this work, the complete genomic sequence of a fish adenovirus, originating from the white sturgeon (Acipenser transmontanus), was determined and analyzed.. Several exceptional features were observed including the longest hitherto known genome size (of 48,395 bp) and a strange location of the putative fiber genes resulting in an unconventional organization pattern.

    Article Title: Discovery of stromal regulatory networks that suppress Ras-sensitized epithelial cell proliferation
    Article Snippet: The PCR products were digested with restriction enzymes and sequentially inserted into the pBluescript II KS(+) phagemid vector (Agilent Technologies; 212207).

    Article Title: L1 retrotransposon-mediated stable gene silencing
    Article Snippet: The neo -cassette tagged L1 RP was inserted into pBluescript II KS(−) phagemid vector (Stratagene, La Jolla, CA) to create pBSKS-L1 RP - neo .

    Article Title: Purification and Partial Characterization of an Entomopoxvirus (DlEPV) from a Parasitic Wasp of Tephritid Fruit Flies.
    Article Snippet: About 10 μg of DlEPV DNA were digested with the EcoRI (RI) restriction enzyme (Roche Molecular Biochemicals) and cloned into the EcoRI site of the pBluescript II KS (+/−) phagemid cloning vector (pBS; Stratagene, www.stratagene.com/ ) using standard protocols ( ).

    Article Title: A Novel Role of RASSF9 in Maintaining Epidermal Homeostasis
    Article Snippet: Fragments corresponding to nucleotides (nt) 285–817 and 1126–1625 of the mouse RASSF9 cDNA (NCBI RefSeq NM_146240) were separately cloned into the Eco RI- Xho I sites of the pBluescript II KS (+) phagemid vector (Stratagene, La Jolla, CA) for riboprobe synthesis.

    Article Title: Antibodies Directed toward Neuraminidase N1 Control Disease in a Mouse Model of Influenza
    Article Snippet: PCR products were purified using the Isolate II PCR and gel kit (Bioline) and cloned in the pBluescript II Phagemid KS+ vector (Agilent Technologies) by blunt-end ligation using T4 ligase (Fermentas).

    Mutagenesis:

    Article Title: Mechanism of Bidirectional Leading-Strand Synthesis Establishment at Eukaryotic DNA Replication Origins
    Article Snippet: pBluescript II KS(-) Phagemid , Agilent Technologies , Cat# 212208-51.

    Article Title: Unconventional gene arrangement and content revealed by full genome analysis of the white sturgeon adenovirus, the single member of the genus Ichtadenovirus.
    Article Snippet: Adenoviruses are commonly found in members of almost every vertebrate lineage except fish and amphibians, from each of which only a single isolate is available as yet.. In this work, the complete genomic sequence of a fish adenovirus, originating from the white sturgeon (Acipenser transmontanus), was determined and analyzed.. Several exceptional features were observed including the longest hitherto known genome size (of 48,395 bp) and a strange location of the putative fiber genes resulting in an unconventional organization pattern.

    Article Title: Discovery of stromal regulatory networks that suppress Ras-sensitized epithelial cell proliferation
    Article Snippet: The PCR products were digested with restriction enzymes and sequentially inserted into the pBluescript II KS(+) phagemid vector (Agilent Technologies; 212207).

    Article Title: L1 retrotransposon-mediated stable gene silencing
    Article Snippet: The neo -cassette tagged L1 RP was inserted into pBluescript II KS(−) phagemid vector (Stratagene, La Jolla, CA) to create pBSKS-L1 RP - neo .

    Article Title: Purification and Partial Characterization of an Entomopoxvirus (DlEPV) from a Parasitic Wasp of Tephritid Fruit Flies.
    Article Snippet: About 10 μg of DlEPV DNA were digested with the EcoRI (RI) restriction enzyme (Roche Molecular Biochemicals) and cloned into the EcoRI site of the pBluescript II KS (+/−) phagemid cloning vector (pBS; Stratagene, www.stratagene.com/ ) using standard protocols ( ).

    Article Title: A Novel Role of RASSF9 in Maintaining Epidermal Homeostasis
    Article Snippet: Fragments corresponding to nucleotides (nt) 285–817 and 1126–1625 of the mouse RASSF9 cDNA (NCBI RefSeq NM_146240) were separately cloned into the Eco RI- Xho I sites of the pBluescript II KS (+) phagemid vector (Stratagene, La Jolla, CA) for riboprobe synthesis.

    Article Title: Antibodies Directed toward Neuraminidase N1 Control Disease in a Mouse Model of Influenza
    Article Snippet: PCR products were purified using the Isolate II PCR and gel kit (Bioline) and cloned in the pBluescript II Phagemid KS+ vector (Agilent Technologies) by blunt-end ligation using T4 ligase (Fermentas).

    Software:

    Article Title: Mechanism of Bidirectional Leading-Strand Synthesis Establishment at Eukaryotic DNA Replication Origins
    Article Snippet: pBluescript II KS(-) Phagemid , Agilent Technologies , Cat# 212208-51.

    Article Title: Unconventional gene arrangement and content revealed by full genome analysis of the white sturgeon adenovirus, the single member of the genus Ichtadenovirus.
    Article Snippet: Adenoviruses are commonly found in members of almost every vertebrate lineage except fish and amphibians, from each of which only a single isolate is available as yet.. In this work, the complete genomic sequence of a fish adenovirus, originating from the white sturgeon (Acipenser transmontanus), was determined and analyzed.. Several exceptional features were observed including the longest hitherto known genome size (of 48,395 bp) and a strange location of the putative fiber genes resulting in an unconventional organization pattern.

    Article Title: Discovery of stromal regulatory networks that suppress Ras-sensitized epithelial cell proliferation
    Article Snippet: The PCR products were digested with restriction enzymes and sequentially inserted into the pBluescript II KS(+) phagemid vector (Agilent Technologies; 212207).

    Article Title: L1 retrotransposon-mediated stable gene silencing
    Article Snippet: The neo -cassette tagged L1 RP was inserted into pBluescript II KS(−) phagemid vector (Stratagene, La Jolla, CA) to create pBSKS-L1 RP - neo .

    Article Title: Purification and Partial Characterization of an Entomopoxvirus (DlEPV) from a Parasitic Wasp of Tephritid Fruit Flies.
    Article Snippet: About 10 μg of DlEPV DNA were digested with the EcoRI (RI) restriction enzyme (Roche Molecular Biochemicals) and cloned into the EcoRI site of the pBluescript II KS (+/−) phagemid cloning vector (pBS; Stratagene, www.stratagene.com/ ) using standard protocols ( ).

    Article Title: A Novel Role of RASSF9 in Maintaining Epidermal Homeostasis
    Article Snippet: Fragments corresponding to nucleotides (nt) 285–817 and 1126–1625 of the mouse RASSF9 cDNA (NCBI RefSeq NM_146240) were separately cloned into the Eco RI- Xho I sites of the pBluescript II KS (+) phagemid vector (Stratagene, La Jolla, CA) for riboprobe synthesis.

    Article Title: Antibodies Directed toward Neuraminidase N1 Control Disease in a Mouse Model of Influenza
    Article Snippet: PCR products were purified using the Isolate II PCR and gel kit (Bioline) and cloned in the pBluescript II Phagemid KS+ vector (Agilent Technologies) by blunt-end ligation using T4 ligase (Fermentas).

    Polymerase Chain Reaction:

    Article Title: Mechanism of Bidirectional Leading-Strand Synthesis Establishment at Eukaryotic DNA Replication Origins
    Article Snippet: pBluescript II KS(-) Phagemid , Agilent Technologies , Cat# 212208-51.

    Article Title: Unconventional gene arrangement and content revealed by full genome analysis of the white sturgeon adenovirus, the single member of the genus Ichtadenovirus.
    Article Snippet: Adenoviruses are commonly found in members of almost every vertebrate lineage except fish and amphibians, from each of which only a single isolate is available as yet.. In this work, the complete genomic sequence of a fish adenovirus, originating from the white sturgeon (Acipenser transmontanus), was determined and analyzed.. Several exceptional features were observed including the longest hitherto known genome size (of 48,395 bp) and a strange location of the putative fiber genes resulting in an unconventional organization pattern.

    Article Title: Discovery of stromal regulatory networks that suppress Ras-sensitized epithelial cell proliferation
    Article Snippet: The PCR products were digested with restriction enzymes and sequentially inserted into the pBluescript II KS(+) phagemid vector (Agilent Technologies; 212207).

    Article Title: L1 retrotransposon-mediated stable gene silencing
    Article Snippet: The neo -cassette tagged L1 RP was inserted into pBluescript II KS(−) phagemid vector (Stratagene, La Jolla, CA) to create pBSKS-L1 RP - neo .

    Article Title: Purification and Partial Characterization of an Entomopoxvirus (DlEPV) from a Parasitic Wasp of Tephritid Fruit Flies.
    Article Snippet: About 10 μg of DlEPV DNA were digested with the EcoRI (RI) restriction enzyme (Roche Molecular Biochemicals) and cloned into the EcoRI site of the pBluescript II KS (+/−) phagemid cloning vector (pBS; Stratagene, www.stratagene.com/ ) using standard protocols ( ).

    Article Title: A Novel Role of RASSF9 in Maintaining Epidermal Homeostasis
    Article Snippet: Fragments corresponding to nucleotides (nt) 285–817 and 1126–1625 of the mouse RASSF9 cDNA (NCBI RefSeq NM_146240) were separately cloned into the Eco RI- Xho I sites of the pBluescript II KS (+) phagemid vector (Stratagene, La Jolla, CA) for riboprobe synthesis.

    Article Title: Antibodies Directed toward Neuraminidase N1 Control Disease in a Mouse Model of Influenza
    Article Snippet: PCR products were purified using the Isolate II PCR and gel kit (Bioline) and cloned in the pBluescript II Phagemid KS+ vector (Agilent Technologies) by blunt-end ligation using T4 ligase (Fermentas).

    Ligation:

    Article Title: Mechanism of Bidirectional Leading-Strand Synthesis Establishment at Eukaryotic DNA Replication Origins
    Article Snippet: pBluescript II KS(-) Phagemid , Agilent Technologies , Cat# 212208-51.

    Article Title: Unconventional gene arrangement and content revealed by full genome analysis of the white sturgeon adenovirus, the single member of the genus Ichtadenovirus.
    Article Snippet: Adenoviruses are commonly found in members of almost every vertebrate lineage except fish and amphibians, from each of which only a single isolate is available as yet.. In this work, the complete genomic sequence of a fish adenovirus, originating from the white sturgeon (Acipenser transmontanus), was determined and analyzed.. Several exceptional features were observed including the longest hitherto known genome size (of 48,395 bp) and a strange location of the putative fiber genes resulting in an unconventional organization pattern.

    Article Title: Discovery of stromal regulatory networks that suppress Ras-sensitized epithelial cell proliferation
    Article Snippet: The PCR products were digested with restriction enzymes and sequentially inserted into the pBluescript II KS(+) phagemid vector (Agilent Technologies; 212207).

    Article Title: L1 retrotransposon-mediated stable gene silencing
    Article Snippet: The neo -cassette tagged L1 RP was inserted into pBluescript II KS(−) phagemid vector (Stratagene, La Jolla, CA) to create pBSKS-L1 RP - neo .

    Article Title: Purification and Partial Characterization of an Entomopoxvirus (DlEPV) from a Parasitic Wasp of Tephritid Fruit Flies.
    Article Snippet: About 10 μg of DlEPV DNA were digested with the EcoRI (RI) restriction enzyme (Roche Molecular Biochemicals) and cloned into the EcoRI site of the pBluescript II KS (+/−) phagemid cloning vector (pBS; Stratagene, www.stratagene.com/ ) using standard protocols ( ).

    Article Title: A Novel Role of RASSF9 in Maintaining Epidermal Homeostasis
    Article Snippet: Fragments corresponding to nucleotides (nt) 285–817 and 1126–1625 of the mouse RASSF9 cDNA (NCBI RefSeq NM_146240) were separately cloned into the Eco RI- Xho I sites of the pBluescript II KS (+) phagemid vector (Stratagene, La Jolla, CA) for riboprobe synthesis.

    Article Title: Antibodies Directed toward Neuraminidase N1 Control Disease in a Mouse Model of Influenza
    Article Snippet: PCR products were purified using the Isolate II PCR and gel kit (Bioline) and cloned in the pBluescript II Phagemid KS+ vector (Agilent Technologies) by blunt-end ligation using T4 ligase (Fermentas).



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    Thermo Fisher pbluescript ii ks (+) phagemid
    M . synoviae strains and plasmids used in this study.
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    Agilent technologies pbluescript ii phagemid ks+ vector
    M . synoviae strains and plasmids used in this study.
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    Image Search Results


    Journal: Molecular Cell

    Article Title: Mechanism of Bidirectional Leading-Strand Synthesis Establishment at Eukaryotic DNA Replication Origins

    doi: 10.1016/j.molcel.2018.10.019

    Figure Lengend Snippet:

    Article Snippet: pBluescript II KS(-) Phagemid , Agilent Technologies , Cat# 212208-51.

    Techniques: Recombinant, Purification, Mutagenesis, Software

    M . synoviae strains and plasmids used in this study.

    Journal: PLoS ONE

    Article Title: Complementation of the Mycoplasma synoviae MS-H vaccine strain with wild-type obg influencing its growth characteristics

    doi: 10.1371/journal.pone.0194528

    Figure Lengend Snippet: M . synoviae strains and plasmids used in this study.

    Article Snippet: A Pst I- Sac II fragment containing vlhA - obg was excised and inserted into the compatible sites of pBluescript II KS (+) phagemid (Thermo Fisher Scientific, Scoresby, Victoria, Australia).

    Techniques: Transformation Assay, Cloning, Expressing, Plasmid Preparation

    (A) Organisation of spo0B operon in B . subtilis and putative obg operon in M . synoviae has been shown. Putative –10 promoter region, transcription start site, ribosome binding site (RBS) of vlhA promoter region, and initiation codon for vlhA gene have been indicated. Length (bp) of each CDS is indicated inside the arrows. Identified stem loop structures in B . subtilis spo0B operon and putative stem loop in M . synoviae ‘ obg operon’ have also been indicated. (B) Schematic presentation of splicing by overlap extension (SOE) PCR to join vlhA gene promoter with obg CDS. Using PCR#1 and PCR#2, vlhA promoter (solid lines) and obg CDS (dotted lines) were amplified using indicated primers. Intermediate products with overlapping fragments (shown by horizontal bars) from both PCRs were amplified by SOE-PCR (PCR#3) using primers vlhA-ExtF and obg-ExtR. (C) Agarose gel electrophoresis of amplification products of vlhA promoter region PCR, obg CDS PCR and SOE-PCR. MW, DNA molecular weight marker (PCR Marker, Sigma, Missouri, USA). (D) Final product of SOE-PCR was first cloned at T-site of pGEM-T Easy Vector and then Pst I- Sac II fragment containing vlhA - obg was inserted between Pst I and Sac II sites of pBluescript II KS (+) vector. Apa I- Sal I restriction fragment containing LoriC and tetM , from pMAS-LoriC plasmid, was cloned at respective sites in pBluescript II KS (+) vector, harboring vlhA - obg , to generate pKS-VOTL plasmid.

    Journal: PLoS ONE

    Article Title: Complementation of the Mycoplasma synoviae MS-H vaccine strain with wild-type obg influencing its growth characteristics

    doi: 10.1371/journal.pone.0194528

    Figure Lengend Snippet: (A) Organisation of spo0B operon in B . subtilis and putative obg operon in M . synoviae has been shown. Putative –10 promoter region, transcription start site, ribosome binding site (RBS) of vlhA promoter region, and initiation codon for vlhA gene have been indicated. Length (bp) of each CDS is indicated inside the arrows. Identified stem loop structures in B . subtilis spo0B operon and putative stem loop in M . synoviae ‘ obg operon’ have also been indicated. (B) Schematic presentation of splicing by overlap extension (SOE) PCR to join vlhA gene promoter with obg CDS. Using PCR#1 and PCR#2, vlhA promoter (solid lines) and obg CDS (dotted lines) were amplified using indicated primers. Intermediate products with overlapping fragments (shown by horizontal bars) from both PCRs were amplified by SOE-PCR (PCR#3) using primers vlhA-ExtF and obg-ExtR. (C) Agarose gel electrophoresis of amplification products of vlhA promoter region PCR, obg CDS PCR and SOE-PCR. MW, DNA molecular weight marker (PCR Marker, Sigma, Missouri, USA). (D) Final product of SOE-PCR was first cloned at T-site of pGEM-T Easy Vector and then Pst I- Sac II fragment containing vlhA - obg was inserted between Pst I and Sac II sites of pBluescript II KS (+) vector. Apa I- Sal I restriction fragment containing LoriC and tetM , from pMAS-LoriC plasmid, was cloned at respective sites in pBluescript II KS (+) vector, harboring vlhA - obg , to generate pKS-VOTL plasmid.

    Article Snippet: A Pst I- Sac II fragment containing vlhA - obg was excised and inserted into the compatible sites of pBluescript II KS (+) phagemid (Thermo Fisher Scientific, Scoresby, Victoria, Australia).

    Techniques: Binding Assay, Overlap Extension Polymerase Chain Reaction, Amplification, Agarose Gel Electrophoresis, Molecular Weight, Marker, Clone Assay, Plasmid Preparation